The latest FastCastTM TGXTM acrylamide master mix has recently been introduced to replace traditional gelling reagents, providing researchers with new gel options. The biggest difference from ordinary acrylamide reagents is that FastCastTM TGXTM reagent gel can withstand 300V separation voltage, which can reduce the traditional SDS-PAGE experiment time from 1-2 hours to 15 minutes under normal conditions, and guarantee the band Clear and sharp. FastCastTM TGXTM Reagent Gel also provides higher transfer efficiency than conventional Tris-HCl gels to ensure high reliability of Western Blot data. FastCastTM TGXTM acrylamide master mix allows the operator to avoid exposure to neurotoxic acrylamide powders and greatly speeds up the experiment and saves lab time.
The FastCastTM TGXTM acrylamide premix kit is usually composed of 4 bottles of reagents: Separation gel A, B and concentrated gels A and B. When making the gel, the AB solution is mixed in equal volume. TEMED and APS can be added to fill the gel. And the step of water/n-butanol pressure can be omitted. The gel is polymerized after 30 minutes and can be used in SDS-PAGE and Western experiments. The prepared gel can also be stored in a refrigerator at 4 ° C and taken at any time within 30 days. The FastCastTM TGXTM acrylamide master mix kit is available in Stain-Free reagents, and the FastCastTM TGXTM acrylamide master mix kit with special fluorescent dyes can be UV-irradiated for 3 minutes on a special imaging system. Similar to the gel image results of the test, the cumbersome test steps are eliminated.
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