Western blot (Western blot). It is an experimental method commonly used in molecular biology, biochemistry and immunogenetics. The basic principle is to color a gel-electrophoresis-treated cell or biological tissue sample by a specific antibody. Information on the expression of a particular protein in the cells or tissues analyzed is obtained by analyzing the location of the staining and the depth of staining.
Western Blot sample preparation should be added with protein lysate (containing protease inhibitor), then ground on ice, collected in EP tube, lysed on ice for 30 min, centrifuged at 4 ° 12000 rpm for 15 min, and the supernatant was dispensed to obtain a protein sample. Direct electrophoresis is recommended, and the extra -80 is saved.
Enzyme linked immunosorbent assay (ELISA) refers to the qualitative and quantitative detection of soluble antigens or antibodies bound to a solid phase carrier such as polystyrene using antigen-antibody binding specificity for immunoreactivity. Enzyme-linked immunosorbent assay (ELISA) is a classic experiment in immunology. This term is introduced in detail from the aspects of definition, basic principle, classification method and operation points.
ELISA samples, direct brain tissue homogenate on ice, collected in EP tube, centrifuged as above, about 220-250 ul per package (because ELISA sample loading is basically 100ul per well, do duplicate holes, 2 holes per sample) , need 200ul, in order to avoid less than 2 holes, when packaging is greater than 200ul each), direct experiment. The extra -80 is saved, as well as avoiding repeated freezing and thawing. However, it is best to pre-test a small number of samples in the ELISA, or consult the literature to see how high the concentration is in the tissue, select the appropriate kit, and the measurement range should be consistent with the tissue concentration. Avoid excessive concentration measurement is not allowed, or the concentration is too low, less than the minimum measurable concentration of the kit.
7.62MM Power+ Signal Power Connector
power connector is used in power module system. It can select the matching power + signal connector according to the need. The feature is that the number of power and signal contacts and the matching sequence can be selected arbitrarily while keeping the connector size and contact core number unchanged.
Plug (male) / socket (female) can be installed at 90 or 180 degrees. It supports mixed or independent combination of signal and power. The quantity range of power and signal is (2-16) pin and (12-128) pin respectively
Product features
High temperature resistant, glass fiber reinforced and flame retardant polyester is used as insulation material
Copper gold composite conductor with high conductivity is used, and the contact area of the conductor is plated with gold
It adopts shrapnel contact, which has the characteristics of integration, small volume, large current carrying capacity, soft plug-in, blind plug-in, self guidance and high dynamic contact reliability. This series of products can be interchanged with FCI's powerblade series and Tyco's multi-beam series
There are three sizes of center distance of power contact: 5.08mm, 6.35mm and 7.62mm
The length of power hole / signal pin can be selected in two sizes. The power rated current is 45A and the signal rated current is 2.5A
7.62MM Power+ Signal Power Connector
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